In Mohs surgery, what is the process followed once a layer of skin is removed?

In Mohs surgery, after a layer of skin is removed, the surgeon performs immediate microscopic analysis of the excised tissue to determine if cancerous cells are present at the margins. If cancerous cells are detected, further layers of skin are systematically removed and analyzed until clear margins are achieved, meaning no cancerous cells are present at the edges of the tissue. This step-by-step process ensures complete removal of the cancerous tissue while preserving as much surrounding healthy tissue as possible. Therefore, the correct answer reflects the critical procedure of continuing to remove layers until clear margins are confirmed, which is essential for the efficacy of Mohs surgery in treating skin cancers.